| Rk | Team | 4x100 |
|---|---|---|
| 1 |
Class 3A - Mississippi Valley-Valley Claire Stuntz, Ava Ramler, Lucy Ehlers, Brynn Walters |
48.90 |
| 2 |
Class 3A - Mississippi Valley-Mississippi Rylee Steffen, Emma Donovan, Kylie Sieverding, Meghan McDonald |
Q 48.97 |
| 3 |
Class 4A - Mississippi Valley-Mississippi Asia Russell, Ryann Decker, Izzy McClure, Sarah Jordan |
49.42 |
| 4 |
Class 4A - Mississippi Valley-Mississippi Amira Coleman, Natalie Ramsey, Jasmine Barney, Alexi Thigpen |
50.36 |
| 5 |
|
50.42 |
| 6 |
Class 4A - Mississippi Valley-Valley Quren Hullon, Ava Kingsbury, Morgan Hospodarsky, Grace Braden |
Q 50.65 |
| 7 |
Class 4A - Mississippi Valley-Valley Safia Almabrazi, Bella Skay, Eva Reynolds, Ani Wedemeyer |
50.95 |
| 8 |
|
Q 50.97 |
| 9 |
Class 4A - Mississippi Valley-Mississippi Samantha Fowler, Gift Gonpue, Brie Meyer, Mimi Kuundu |
Q 50.98 |
| 10 |
Class 4A - Mississippi Valley-Mississippi Sophia Mason, Paige Paup, Avalon Burns, Avery Berte |
51.33 |
| 11 |
Class 4A - Mississippi Valley-Valley Akeelah Anderson, Savannah Bailey, Mallory Tomkins, Addison Wright |
52.00 |
| 12 |
Class 4A - Mississippi Valley-Valley Natalie Steva, Eriniah Carter, Shaniya Jackson, Annie Schwartz |
52.34 |
| 13 |
Class 4A - Mississippi Valley-Valley Sage Slessor, Kamarionna Crittenden , Ava Pasker, Cassie Thurmond |
52.43 |
| 14 |
Class 4A - Mississippi Valley-Valley Alexa Zanders, Charlotte Gettman, Samantha Elliott, Jada Smith |
52.84 |
| 15 |
Class 4A - Mississippi Valley-Mississippi Olivia Brimeyer, Catharine Ruden, Ava Singletary, Jenna Lawler |
53.34 |
| 16 |
Lindsey McKane, Evelyn Asay, Shaniya Jackson, Natalie Steva |
54.37 |
| 17 |
Kelsey Kaut, Hailey Noel, Haley Schroeder, Hannah Ramsey |
54.43 |
| 18 |
Aaliyah Hoskins, Taylor Brejcha, Tasia Dietz, Ella Neil |
55.85 |
| 19 |
Tristan Jones, Elise Gatto, Kaydence Ahart, Ava Ahart |
58.14 |
| 20 |
Dede Aboussa-Folly, Lila Howe, Amal Eltayib, Paige Dixon |
59.17 |
Mississippi Valley