| Rk | Team | 4x100 |
|---|---|---|
| 1 |
Class 4A - Mississippi Valley - Valley Division Miles Parlet, Miles Thompson, Noah Johnson, Michael Blank |
41.71 |
| 2 |
Class 4A - Mississippi Valley - Valley Division Todd Rent, Izaiah Loveless, Daniel Robinson, Christian Janis |
41.89 |
| 3 |
Class 4A - Mississippi Valley - Mississippi Division Daunte Washington, sulemani samuel, Raquis Robinson, Zack Robinson |
Q 42.56 |
| 4 |
Class 3A - Mississippi Valley - Valley Division Kaleb Rowland, Drew Burds, Grant Glausser, Brock Carpenter |
42.65 |
| 5 |
Class 4A - Mississippi Valley - Valley Division Anthony Franklin, Tre Duffie, Donovan Slick-Driscoll, Jacob Doyle |
Q 42.69 |
| 6 |
Class 4A - Mississippi Valley - Mississippi Division Mitchell Darst, Conner Trunnell, Jacob Kieler, William Remmert |
Q 42.89 |
| 7 |
Class 4A - Mississippi Valley - Mississippi Division Quinton Alexander, Sam Asiedu-Okyere, Nate Patterson, Keegan Erickson |
Q 43.00 |
| 8 |
Class 4A - Mississippi Valley - Mississippi Division Deyon Moore, Nick Lambe, Jaden Arnold, Benjamin Francis |
43.04 |
| 9 |
Class 4A - Mississippi Valley - Valley Division Nathan Leemhuis, Tayden Ferguson, Carter Henderson, Kaden McCloe |
Q 43.20 |
| 10 |
Class 4A - Mississippi Valley - Valley Division Deon Brown, Ronnie Major, Phillip Kaplan, Sam Rew |
Q 43.21 |
| 11 |
Class 4A - Mississippi Valley - Mississippi Division Aiden Maher, Carter Meyers, Jalen Wieck, Da'Shon Fisher |
Q 43.22 |
| 12 |
Class 4A - Mississippi Valley - Valley Division Ben Frazier, DeSean Kemp, Nate Kline, Cavelle Fay |
Q 43.29 |
| 13 |
Class 3A - Mississippi Valley - Mississippi Division Joseph Casey, Grayson Hartman, Coleton Beasler-Weber, Aidan McDermott |
43.82 |
| 14 |
Class 3A - Mississippi Valley - Mississippi Division William Herbst, Drew Reilly, Matthew Nachtman, Ryan Brosius |
44.41 |
| 14 |
Alvin Gweh, Chauncey Bodden, Dawson Dougherty, Jacob Doyle |
44.41 |
| 16 |
Class 4A - Mississippi Valley - Valley Division Brendon Zheng, Justin Potts, Steffonte Jones, Jacob Johnson |
44.80 |
| 17 |
Chase Eisenbach, Sutton Koller, Ben O'Connor, Lucas Meyer |
46.26 |
| 18 |
Cooper Gonzalez, Carter Northey, Connor Donaldson, Camden Henderson |
46.60 |
| 19 |
Canaan Barnes, Zion Shaw, Jack Wallace, Jay'den Williamson |
47.77 |
| 20 |
Thierry Boyd, Anthony Petit-Frere, Antonis Christodoulou, Ali Mwalimu |
48.33 |
Mississippi Valley